Genetic examinations list

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Cystic fibrosis – 50 mutations + Tn variants of IVS8 in the CFTR gene

Testing for the 50 most common CFTR gene mutations and determination of polymorphism in intron 8 (IVS8 Tn/TGn). These 50 mutations represent over 92% of the findings in patients diagnosed with CF in the Czech population using fragmentation analysis.

Material:

Peripheral blood, Isolated DNA from blood

Turnover Time:

3 weeks

STATIM

1 week

Cystic fibrosis – 50 mutations + Tn variants of IVS8 in the CFTR gene

Testing for the 50 most common CFTR gene mutations and determination of polymorphism in intron 8 (IVS8 Tn/TGn). These 50 mutations represent over 92% of the findings in patients diagnosed with CF in the Czech population using fragmentation analysis.

Material:

Peripheral blood, Isolated DNA from blood

Turnover Time:

3 weeks

STATIM

3 days

Cascade prenatal testing (by QF-PCR, array) excluding maternal contamination

Cascade aneuploidy testing of material after invasive prenatal examination using QF-PCR and array methods, excluding maternal contamination. This can be supplemented by foetal karyotyping, if necessary.

Material:

Chorionic villi, Amniotic fluid …
Chorionic villi, Amniotic fluid, Cord blood, Isolated DNA from chorionic villi, Isolated DNA from amniotic fluid, Isolated DNA from cordocentesis, DNA isolated from cultured cells

Turnover Time:

3 weeks

Cascade testing of the product of conception (by QF-PCR methods) excluding maternal contamination

Cascade aneuploidy testing of aborted foetal tissue using QF-PCR, arrays or karyotyping; excluding maternal contamination.

Material:

Conception product, DNA from the product of conception

Turnover Time:

3 weeks

SOD1 gene testing – familial amyotrophic lateral sclerosis (ALS)

Testing for all coding exons (1, 2, 3, 4, 5) including adjacent SOD1 gene intron sequences by Sanger sequencing.

Material:

Peripheral blood, Buccal swab …
Peripheral blood, Buccal swab, Isolated DNA from blood, Cultured cells

Turnover Time:

3 weeks

STATIM

1 week

PABPN1 gene testing (exon 1) – oculopharyngeal muscular dystrophy (OPMD) – familial AD

PCR amplification and sequencing of PABPN1 gene (associated with oculopharyngeal muscular dystrophy) to detect GCG triplet expansion.

Material:

Peripheral blood, Buccal swab …
Peripheral blood, Buccal swab, Isolated DNA from blood, Isolated DNA from chorionic villi, Isolated DNA from amniotic fluid, Isolated DNA from cordocentesis, DNA isolated from the product of conception, Cultured cells

Turnover Time:

3 weeks

STATIM

1 week